A rabbit jejunal isolated enterocyte preparation suitable for transport studies.
نویسندگان
چکیده
منابع مشابه
Ion Transport in Isolated Rabbit
The addition of actively transported sugars to the solution bathing the mucosal surface of an in vitro preparation of distal rabbit ileum results in a rapid increase in the transmural potential difference, the short-circuit current, and the rate of active Na transport from mucosa to scrosa. These effects arc dependent upon the active transport of the sugar per se and arc independent of the meta...
متن کاملIon Transport in Isolated Rabbit Ileum
The transmural potential difference, short-circuit current, and Na fluxes have been investigated in an in vitro preparation of isolated rabbit ileum. When the tissue is perfused with a physiological buffer, the serosal surface is electrically positive with respect to the mucosal surface and the initial potential difference in the presence of glucose averages 9 mv. Unidirectional and net Na flux...
متن کاملLysine Transport across Isolated Rabbit Ileum
Lysine transport by in vitro distal rabbit ileum has been investigated by determining (a) transmural fluxes across short-circuited segments of the tissue; (b) accumulation by mucosal strips; and (c) influx from the mucosal solution across the brush border into the epithelium. Net transmural flux of lysine is considerably smaller than that of alanine. However, lysine influx across the brush bord...
متن کاملMultinuclear NMR Studies of Isolated Rabbit Hearts
The hypothesis that substrate availability can alter contractile function in reperfused myocardium after global ischemia was investigated in this study. Isolated rabbit hearts were placed in a dual tuned C'PI13C) NMR probe with a 9.4-T magnet and perfused with the following substrates given individually or in combination: 10 mM glucose, 2 mM palmitate, and 2.5 mM [3-13C]pyruvate. Glucose was th...
متن کاملPreparation of isolated multiple forms of rabbit brain aldolase.
1. A method is described for partial purification of the total aldolase of rabbit brain by ammonium sulphate precipitation followed by fractionation of the aldolase into five electrophoretically distinct forms. 2. The method allows a laboratory-scale preparation of the five aldolases. 3. With the exception of band 3 the fructose 1,6-diphosphate/fructose 1-phosphate activity ratios of the isolat...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1985
ISSN: 0022-3751
DOI: 10.1113/jphysiol.1985.sp015708